Journal: International Journal of Nanomedicine

Article Title: Human serum albumin-based probes for molecular targeting of macrophage scavenger receptors

doi: 10.2147/IJN.S197990

Figure Lengend Snippet: Evaluation of Mal-HSA-FITC uptake by different cell types. Fluorescence microscopy images of Mal-HSA-FITC probes (green) incubated for 3 h with ( A ) THP-1 macrophages, ( C ) HUVECs (EC), and ( D ) HCtSMCs (SMC). ( B ) Mal-HSA-FITC uptake by different cell types plotted against time. The results represent data from technical triplicates (9 images/well and timepoint) and are presented as mean and SEM. Data show significantly increased uptake of modified HSA probes by THP-1 cells compared with HUVEC (**** P <0.0001) and HCtSMCs ( #### P<0.0001) at 2.5 h and 3 h. None of the cell types recognized HSA-FITC (data not shown) (repeated measures two-way ANOVA (factors: cell type and time) and Tukey’s multiple comparison test). Abbreviations: HSA, human serum albumin; GCU, green calibration unit.

Article Snippet: Human carotid smooth muscle cells (HCtSMCs) (Cell Applications, San Diego, CA, USA) were cultured up to passage 7 in human smooth muscle cell media (Cell Applications).

Techniques: Fluorescence, Microscopy, Incubation, Modification, Comparison

Journal: Cancers

Article Title: Mambalgin-2 Induces Cell Cycle Arrest and Apoptosis in Glioma Cells via Interaction with ASIC1a

doi: 10.3390/cancers12071837

Figure Lengend Snippet: qPCR analysis of Acid-sensing Ion Channels (ASICs) and epithelial Na + channels (ENaCs) mRNA expression in U251 MG and A172 cells and normal astrocytes. Gene expression was normalized to the β- ACTIN , GPDH and RPL13a housekeeping genes and presented as lg of relative mRNA level ± standard error of mean (SEM) ( n = 3–5).

Article Snippet: Human astrocytes (Cell Applications, San Diego, CA, USA) were grown in Neurobasal A medium (Thermo Fisher, Waltham, MA, USA) with addition of G-5 (PanEco) Cells were maintained at 37 °C in a humidified atmosphere with 5% CO 2 .

Techniques: Expressing, Gene Expression

Journal: Cancers

Article Title: Mambalgin-2 Induces Cell Cycle Arrest and Apoptosis in Glioma Cells via Interaction with ASIC1a

doi: 10.3390/cancers12071837

Figure Lengend Snippet: Mambalgin-2 influence on growth of U251 MG and A172 glioma cells and normal astrocytes: ( a ) 1 µM mambalgin-2 effect on U251 MG, A172 cells and astrocytes proliferation during different incubation times. Data are presented as % of control (untreated cells, dashed line) ± SEM (n = 4). # ( p < 0.05), ## ( p < 0.01), and ### ( p < 0.001) indicate significant difference from the control according to One-way ANOVA followed by Dunnett’s test; ( b ) Influence of 1 μM mambalgin-2 and its Leu32Ala and Leu34Ala mutants on proliferation of U251 MG and A172 cells during 72-h incubation. Data are presented as % of control (untreated cells, dashed line) ± SEM (n = 4). ### ( p < 0.001) indicates significant difference from the control according to One-way ANOVA followed Dunnett’s hoc test. *** ( p < 0.001) indicates significant difference in activity of mutants and mambalgin-2 according to One-way ANOVA followed by Dunnett’s test; ( c , d ) effect of different mambalgin-2 and amiloride concentrations on U251 MG ( c ) and A172 ( d ) cell growth during 72-h incubation. The parameters describing concentration-effect curves (EC 50 , A 0 ) are given in the . Data are presented as % of control (untreated cells) ± SEM ( n = 3–11); ( e ) analysis of the pH value of culture medium during prolonged cultivation of U251 MG and A172 cells ( n = 4). ** ( p < 0.01) and *** ( p < 0.001) indicate significant difference from the pH value of the culture media in an initial time point according two-tailed t -test.

Article Snippet: Human astrocytes (Cell Applications, San Diego, CA, USA) were grown in Neurobasal A medium (Thermo Fisher, Waltham, MA, USA) with addition of G-5 (PanEco) Cells were maintained at 37 °C in a humidified atmosphere with 5% CO 2 .

Techniques: Incubation, Control, Activity Assay, Concentration Assay, Two Tailed Test

Journal: Cancers

Article Title: Mambalgin-2 Induces Cell Cycle Arrest and Apoptosis in Glioma Cells via Interaction with ASIC1a

doi: 10.3390/cancers12071837

Figure Lengend Snippet: Mambalgin-2 effect on cells of primary culture obtained from a patient with glioblastoma (GBM). ( a ) Analysis of ASICs and ENaCs gene expression in primary GBM cells by qPCR. Gene expression was normalized to the β- ACTIN , GPDH and RPL13a housekeeping genes and presented as lg of relative mRNA level ± SEM ( n = 3–5); ( b ) Influence of different mambalgin-2 concentrations on viability of primary GBM cells. Cells were incubated for five days with mambalgin-2 and their viability was accessed by Alamar Blue assay. Data are presented as % of control (untreated cells) ± SEM (n = 3); ( c ) Comparison of the 50 µM mambalgin-2 inhibitory effect on viability of normal astrocytes, U251 MG, A172, and primary GBM cells by Alamar Blue assay. Data are presented as % of control (untreated cells, dashed line) ± SEM (n = 3). ## ( p < 0.01) and ### ( p < 0.001) indicate significant difference between treated and untreated cells according to One-way ANOVA followed by Dunnett’s test. *** ( p < 0.001) indicates significant difference of mambalgin-2 action on cells from the effect on astrocytes according to One-way ANOVA followed by Dunnett’s test.

Article Snippet: Human astrocytes (Cell Applications, San Diego, CA, USA) were grown in Neurobasal A medium (Thermo Fisher, Waltham, MA, USA) with addition of G-5 (PanEco) Cells were maintained at 37 °C in a humidified atmosphere with 5% CO 2 .

Techniques: Gene Expression, Incubation, Alamar Blue Assay, Control, Comparison

Journal: FASEB BioAdvances

Article Title: Age and Sex Impact the Role of Thrombospondin‐2 and Thrombospondin‐5 in Response to Hindlimb Ischemia

doi: 10.1096/fba.2025-00258

Figure Lengend Snippet: TSP‐2 inhibits angiogenesis in early passage ECs, and TSP‐5 may be protective against tubule disruption. (A, B) Angiogenesis was assessed by plating ECs overnight on a Matrigel‐based endothelial tubule formation assay and pretreating with or without TSP‐2 (5 μg/mL) or TSP‐5 (5 μg/mL). TSP‐2 inhibited tubule formation in early passage ECs, but TSP‐5 inhibited tubule formation in late passage ECs. (C, D) To assess tubule disruption, EC tubules were allowed to form overnight and then treated for 24 h with TSP‐2 (5 μg/mL) or TSP‐5 (5 μg/mL). TSP‐2 inhibited tubule formation in late passage ECs (D), TSP‐5 increased tubule formation in early passage ECs (C). In the postformation treatment groups, late passage ECs had more tubule disruption than early passage cells. Values are means ± SE. Student's t ‐test performed for analysis, * p < 0.05 compared to nontreatment.

Article Snippet: Human aortic endothelial cells (Cell Applications Inc., San Diego, CA, USA) were used in early passage (P3–P8) to represent young cells or late passage (P15–P18) to represent aged cells.

Techniques: Disruption, Tube Formation Assay